Thuong Trinh-Minh

4 Questions 2 Answers 0 Followers

Questions related from Thuong Trinh-Minh

Seeking advice on single-cell isolation from RNAlater-fixed tissues for scRNAseq?

Hi everyone, I am currently working with mouse skin and lung tissues preserved in RNAlater (Invitrogen). However, I have limited experience with single-cell RNA sequencing (scRNAseq). I would...

24 May 2025 1,947 1 View

Could somebody help me with RANKL-stimulated Osteoclast from RAW 264.7 (Sigma)?

Hi everyone, I am doing osteoclast stimulation from RAW cell, passage 5. I seeded 2500, 5000 and 10000 cells in 96 well-plate with RANKL from R&D (20 and 40ng/mL), MCSF (25ng/mL) and change...

27 April 2017 7,048 2 View

Whole cell lysis buffer for Co-IP?

Hi everyone, I plan to analyze protein-protein interaction in my cell by Co-IP. However, when I check on Internet, my lysis buffer could not lyse nuclei. Could anyone possibly suggest me what...

04 January 2017 5,833 3 View

Does anybody use Guide-it CRISPR/Cas9 Gesicles from TAKARA brand?

I searched some CRISPR/Cas9 system and I found that Guide-it CRISPR/Cas9 Gesicles from TAKARA company (Clontech) is somehow really fancy. Does anyone use this system before? Could you possibly...

02 January 2017 5,162 1 View