What is the best treatment to block autophagy in C2C12 cell line?

I read that NH4CL and chloroquine treatments can be used to block autophagic flux. Are there any other acceptable treatments?

02 September 2020 4,252 3 View

How to generate heterozygous knockouts using CRISPR Cas9 in iPSCs?

Hi all. I'm trying to create a heterozygous deletion (55bp) mutation in iPSCs to model malignant tranformation. I'm using two sgRNAs in a plasmid based system (puromycin selection). I obtained ~20...

18 May 2020 5,389 1 View

Are there guidelines for conducting a meta analysis and scoping review on case reports?

Currently, I can only find case reports on the rare condition I am planning to conduct a meta-analysis on. I will be evaluating outcomes and efficacy of treatments. Are there any known guidelines...

24 February 2020 5,742 3 View

5x5 crosstabs table on spss, iteration keeps going up and no results , anyone know why?

Hey all, So im trying to run 5X5 crosstabs with fisher exact test on SPSS, got about 1000~ lines in the file. Im seeing the iteration number keeps going up (1700 currently), but nothing happens...

07 December 2018 5,602 4 View

PVA freezing/thawing cycles - when is it better to "pause"?

I am currently working with PVA using the physical polymerization method, of multiple freezing/thawing (F/T) cycles. Since I've been getting opposite opinions regarding this question, I wanted to...

25 February 2017 2,797 1 View

How to oxidize Nitric oxide?

20 June 2016 2,008 9 View

Where can I purchase Silica nanoparticles?

In the past I worked with silica nanoparticles bought from "Nissan Chemicals", Product No. ST-40, see link for other types.In the company site I can't find that silica anymore.How can I get that...

17 August 2014 6,289 10 View

What are the best ADHD diagnostic methods, given that my goal is to associate between risky driving and sub-impairments (or sub-dimensions) of ADHD?

Hi, I am studying the links between ADHD sub-dimensions and the different types of risky driving. What are the best ADHD diagnostic methods, given that my goal is to associate between risky...

01 January 1970 2,805 1 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View