Seeds of wild Brassica rapa?

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Fortran or julia?

I am currently working on improving one of my code and considering transforming my Fortran code to Julia. In your experience, what are the potential benefits of converting Fortran code to Julia?...

17 July 2023 5,434 2 View

How do you publish your research here?

How do you publish your research here

07 June 2023 7,482 2 View

How do I calculate shear rate conversion factor for Anton Paar ViscoQC 100 Measuring system cc18?

Its manual doesn't have the conversion factor as Brookfield's does. I wish to convert rpm to shear rate but I don't know how.

08 April 2023 4,351 1 View

How to purify cytokines from mouse ear sample?

Is there anybody out there who has experience how to isolate and purify common inflammatory cytokines (TNFa, IL-1b, IL-6 etc.) from inflamed mouse ear tissue? We usually do it with blender using...

29 March 2023 4,807 1 View

Sequencing error or quasispecies?

Hi! I amplified overlapping PCR products of the same viral genome region from the same sample using two different primer sets. I sequenced both products and there's one nucleotide difference in...

14 February 2023 4,690 5 View

Perception Of Agric science and Chemistry Teachers on the relevance of agriculture chemistry in effective food production?

I'm working on a project with this topic and this is my first time and I don't know how to go about it

14 December 2021 6,718 6 View

How to perform a good extraction of calprotectin from feces ?

Hi, I would like to test a new commercial ELISA kit on fecal calprotectin but it is for serum. How can I extract fecal calprotectin to obtain a liquid clear solution ? Thank a lot for all...

07 December 2021 553 2 View

Would you know a free software to draw schema for scientific papers?

I would like make a figure in which could be chosen plants, soil, roots and other parameters. Power point is quite limited for scientific drawing. Would you have better option to propose?

06 May 2021 4,698 17 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 7,067 5 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,728 2 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How should I prevent mould contamination on small seeds in a petri dish exposed to warm temperatures?

Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...

24 July 2024 8,850 4 View

HEC 1A & HEC1B Cell Lines?

Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...

20 July 2024 4,143 2 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

How to convert protein percentage into kg/ha?

I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.

11 July 2024 9,045 4 View

How can I determine the percentage of cell viability of cells seeded in 96 well plate ?

I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...

10 July 2024 7,145 2 View

I seed my cell in Opti-MEM?

I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...

07 July 2024 407 0 View

Do the SHSY5Y cells tend to form multiple layers over each other when highly confluent?

I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.

01 July 2024 8,361 3 View

How to transfer a full grown cell monolayer to another plate?

Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...

30 June 2024 9,300 0 View