How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Which proteins are continuously expressed (at stable levels) in mammalian monocytes and thus, suitable controls for Protein expression studies?

Hello! Currently I am testing whether an externally added agent affects the expression of only one particular protein or whether it generally inhibits protein synthesis due to its toxicity. For...

14 September 2020 6,359 3 View

Suggestions for an easy experiment on an inverted fluorescence microscope (students lab course)?

09 September 2020 290 3 View

Why it is that Disulfiram (DSF) only binds to zinc in cancer cells?

Good Afternoon, I understand that the zinc transporter proteins (ZIP7 and ZIP10) are both present in healthy and diseased cells. I learnt that zinc uptake is elevated in malignant cells...

09 October 2019 5,069 3 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Compare multiple means over time, which analysis?

I have a repeated measures design, looking at how sex hormones effect number of responses on a dichotic listening task, three times over the course of the menstrual cycle. Where it simply the...

14 April 2019 8,108 3 View

What is the best way to extract microRNA in postprandial blood?

Because of lipids in postprandial blood, I have some troubles to extract microRNA. How can I remove lipids without alter the quality of microRNA?

25 February 2019 2,781 3 View

What is the best way to know the quality of RNAm from plasma?

Hi everybody, I did extraction of total RNA on plasma samples (I am interested in circulating microRNA). How can I do to know if my sample are of good quality and if there are no...

01 October 2018 4,786 2 View

Why do I get different kind of staining with a same antibody in cryosections compared to paraffin sections by immunofluorescence?

I am looking at the expression of a particular protein in the skin by immunofluorescence and I would like to know which cells express this protein. For that, I stained both cryosections and...

20 February 2017 7,094 1 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View