I am a M.Phil Bioscience student who currently testing the antibacterial susceptibility test using plant extract toward S. mutans, a cariogenic bacteria that cause dental caries. I found out there is formation of sediment on the 96-well plate after performing broth microdilution test. This sedimentation hinders the light ray to pass through and interfere with microplate reading measurements. Can anyone suggest way to solve this problem?
By employing centrifugation, filtration, homogenization, and optical clearing, you can potentially mitigate sediment formation in the 96-well plate during your antibacterial susceptibility tests. This approach ensures clearer readings and more accurate results in your research on the efficacy of plant extracts against S. mutans.