Hello. I am learning about different types of cell transfection methods, and I can't find out why protocols and descriptions only explain DNA transfection with calcium phosphate and not RNA or other nucleic acids. What is the reason for this? Thanks.
There are papers that use the method for RNAi but they tend to be cancer focused (eg. https://www.sciencedirect.com/science/article/pii/S0168365906000368). Do not forget about CPP tagged VLPs.
In reply to your question:
"Can you use calcium phosphate cell transfection for RNA delivery?"
Based on the following publications, the answer is yes:
Importance of divalent cations in nanolipoplex gene delivery.
MR Mozafari, A Omri
Journal of Pharmaceutical Sciences 96 (8), 1955-1966 (2007).
and:
Mechanism of calcium ion induced multilamellar vesicle-DNA interaction.
MR Mozafari, V Hasirci
Journal of Microencapsulation 15 (1), 55-65 (1998).
and:
Supramolecular assemblies of zwitterionic nanoliposome-polynucleotide complexes as gene transfer vectors: Nanolipoplex formulation and in vitro characterisation.
MR Mohammadabadi, M El-Tamimy, R Gianello, MR Mozafari
Journal of liposome research 19 (2), 105-115 (2009).
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