Why is ccdb toxin not killing susceptible strains of E.coli?

Hi hive, We are trying to use ccdb as a counter selection marker. We are using the cassette from this paper, which should be highly expressed: A new method to customize protein expression vectors...

22 June 2020 5,084 5 View

Appropriate statistical test for within-group comparisons of questionnaires?

Dear colleagues, I am trying to determine the appropriate statistical test for my problem. I have read several discussions on the topic here, however was unable to extract an answer, and so I...

03 May 2020 6,281 10 View

How to interpret transition states whose Gibbs-energies are higher than the Gibbs-energies of their corresponding ground states?

I am performing DFT-calculations (gamess-us) on a reaction: ground state 1 -> transitition state -> ground state 2. (in short: gs1 -> ts -> gs2.) ts is only 0.3 kcal/mol higher in...

16 February 2020 2,261 1 View

Open datasets for Machine Learning in geotechnics / engineering geology?

Machine learning has proven to be very successfull in many disciplines and the general development is surely furthered by openly accesible algorithms and datasets for the whole community to work...

23 October 2019 6,941 19 View

Is it possible to produce a device that can project screen out of the mind in physical - how is the theory of that?

Can there exist societysystem including projections of pictures and video frames out of thought into the physical reality? Is this possible for nature of human beeings or has this to be technical...

30 May 2019 2,426 5 View

How to convert the fracture toughness to strength?

How to convert the fracture toughness to strength?

16 December 2018 2,438 32 View

Young's modulus of tetragonal and cubic zirconia?

For a tetragonal zirconia, is it 210 GPa? And for cubic?

24 November 2018 9,064 2 View

The correctness of the calculation of fracture toughness of ceramics via Vickers indentation method?

30 October 2018 523 4 View

How to conduct power and sample size calculations for the probit model with binary covariate(s)?

I would like to calculate the optimal sample size (based on power calculations) for the probit model and a binary covariate.I have run a pilot study for a randomized control trial. I am interested...

03 September 2017 5,895 3 View

Hydrophilicity of PDMS due to treatment with Piranha and KOH. Treatment time?

Apart from plasma activation, PDMS can be made hydrophilic by treating it with Piranha solution (and maybe KOH in an elaborate approach). I found two papers reporting this effect. Interestingly,...

18 April 2017 1,857 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View