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What's the better material instead of Glutamine for CHO cells in cell culture?

I've designed media for CHO cells, without Glutamine! But when they're in large scale, the cells were damaged and decreased their viability. What's your refer ?

14 January 2024 2,503 2 View

How can I find PCR markers and suitable antibody to flow cytometry of C2C12 cell line?

How can I find "PCR markers" and "suitable antibody to Flow cytometry" of C2C12 cell line? Is there any web server to find this data?

02 December 2023 3,248 0 View

Can someone provide me with the earthquake repositioning code with Python?

29 September 2023 6,469 3 View

Would using a high ROX master mix cause any issue for PCR instruments that do not require ROX as normalizer?

Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...

22 July 2023 964 6 View

How long can transient plasmids (like PEGFP N1) remain after transfection into bone marrow stem cells?

hello, I have a major question that i would really appreciate if anyone could make me clear. In a project I am going to transfect BMSCs with a plasmid by lipofectamine 2000 reagent. then the...

06 June 2023 9,768 1 View

Can I import bipolar electrode montage into eeglab?

I want to use an epilepsy database ( CHB-MIT Scalp EEG Database) and the samples have some bipolar channels like FP1-F7, F7-T7, T7-P7, P7-O1, FP1-F3, etc. We can define monopolar channels in...

12 July 2022 3,546 0 View

Can we extract intracellular cellulose from bacteria which exist in paper mill activated sludge?How?

I dont mean bacterial cellulose(the cellulose which produce by bacteria like G.xylinus ) or the cellulose fibril that present in paper mill activated sludge, i mean a protocol for extracting the...

02 July 2022 6,182 2 View

Is there any fully automatic MR prostate segmentation?

Is there any free available tool or even demo that gets the MRI image and creates the contouring of different organs? I need to just test some few cases. Thanks.

07 March 2022 1,689 0 View

How to mature "Oligodendrocyte precursor cells (OPCs)" CELL-LINE to "Myelin producing Oligodendrocytes"?

Hello everyone I am working on a human cell-line of Oligodendrocyte Precursor cell (OPCs) called MHB. Results of my immunocytochemistry experiments showed that these cells express NG2, and don’t...

15 December 2021 3,208 1 View

With the NCBI Probe Database being retired, is there any similar database I can refer to for finding tetra nucloetide microsatellite markers?

I have been searching the publications reporting STR markers in the genomic neighborhood of my interest, but I wonder if there might be a more straightforward approach to it. Also within the UCSC...

23 November 2021 8,236 4 View

Posting a research survey ?

Hi i want to post this in various groups, how do i do that ? hey, please do my survey when you get the chance, it will take 15 minutes tops x I am looking for UK secondary school teachers to...

30 June 2024 9,594 2 View

Experience Sampling Method (ESM) Solutions for Real-Time Voice Data Collection?

Hi Everyone, I am working on a research project focused on analyzing behavior and cognition in different environments and situations. I am looking for a solution that allows us to: Record...

29 June 2024 6,540 0 View

Difference between Distribution System Operator and Distribution Network Operator?

There is a lot of confusion between the Distribution System Operator (DSO) and the Distribution Network Operator (DNO). Please clarify the roles and responsibilities of the DSO and DNO. Are these...

20 June 2024 7,381 0 View

Should a researcher seek permission from the author of a research instrument accessed online?

I found this online research tool which is relevant to my research project. Is it safe to use it without the author's consent or should I seek first his permission before using the instrument?

19 June 2024 603 0 View

Can dating detrital zircon in thin sections affect the representativeness of dates?

Dear Geology Community, I am currently researching the dating of detrital zircon in metasediments. Due to the small size of my samples, traditional mineral separation methods may not yield a...

09 May 2024 337 7 View

What are the gaps surrounding Gig work in Africa [specifically Kenya]?

I've been dabbling into gig work in Kenya recently. I understand that there isn't much literature on it. What gaps do you see in that space of gig work?

04 May 2024 2,294 0 View

Should there be a BS in 'Doctor of Psychotherapy' degree to meet the growing demand for mental health problems??

"Should there be a BS in 'Doctor of Psychotherapy' degree to meet the growing demand for mental health problems?? and if so, what might such a program entail?" While "Doctor of Physiotherapy" is...

10 April 2024 7,083 3 View

How can one use GIS-based techniques for site suitability analysis of Continuously Operating Reference Stations (CORS)?

In an area or region where there are inadequate Continuously Operating Reference Stations (CORS), how can one use GIS based techniques such as Multi - Criteria Decision modelling to analyze site...

27 March 2024 10,013 1 View

What impact does the integration of Augmented Reality (AR), Virtual Reality (VR), and the Metaverse have on the Online Learning?

Please Answer using AI, VR, AR is good in Online education. Blended learning is good? Compare the with traditional education.

26 February 2024 4,507 5 View

How can one gather 2/5-years old tweets from X?

Hi ResearchGate community, I am a political theorist (limited coding skills) and in my research I am looking at an online campaign occurred between 2019-2021. I would like to gather the tweets...

22 February 2024 1,516 0 View

Negar Sioofy

Many thanks Ossama Mohamed Zakaria

Michael Sean Molloy

Hi Negar

You can use AND only, the more of these Boolean operators you use the narrower your search will become

Quite lot of information at the following link

https://learn.nlm.nih.gov/rest/training-packets/T0042010P.html

Mick

Thank you dear Michael for your helpful information