I have plan to grow tumor subcutaneously into flank location in immunodeficient mice. Can anyone please let me clear that which immunodeficient mice (SCID/Nude) are better to determine the in vivo antitumor activity of CAR T cells. Moreover, also advise me that direct intratumoral injection is more effective or intravenous tail vein.
In SCID mice recessive mutation in 16 chromosome responsible for deficient activity of Prcdc enzime involved into DNA repair takes place. This really results in deficiency in V(D)J recombination, but not absolute absence of T and B cells. Therefore, experimental models using SCID mice are at some extent "leaky". So, study performed on SCID mice may result in hardly interpretable results. It would be better to use RAG knockout mice. In your case, the absence of mature T cells should be critical. So, you can successfully use young (!) Nude mice. In old Nude mice some quantity of mature T cells can be differentiated.
There have been significant advances in the field of immunodeficient mice. These two reviews explain the advantages of some of the more advanced models:
Article Humanized Mouse Models for the Preclinical Assessment of Can...
Article Humanized Mice for the Study of Immuno-Oncology
You'll probably be interested in this paper from Cellectis using the BRGS model:
https://www.nature.com/articles/s41598-018-27264-w
Dmitry B. Kazansky and Mark Livingstone thank you so much for adding answer.
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