How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How to calculate dilution factor based on the highest OD/concentration of sample in ELISA?

I am using Elabscience sandwich ELISA kit (detection range 0.31-20ng/ml), I am using supernatant saliva samples. I am using 4 plates and plotting the standard curve for every plate. In one of the...

27 January 2021 9,759 1 View

Which compounds might cross-react with cortisol in Elisa kit?

Hi, I'm analyzing cortisol in post smolt salmon with two elisa kits. The first is made for human saliva (Fairly cheap) and the second one for fish (three times the price). I was hoping to get...

21 January 2021 6,874 1 View

What is optimal buffer for saliva immunoglobulins testing with lateral flow immunoassay?

Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...

16 November 2020 1,520 3 View

Quick way to collect saliva to study viral RNA expression?

Hello, I am going to perform some saliva's RNA analysis. I found in the litterature several protocols to collect and prepare saliva for RNA/DNA analysis. For main of them we have to use kits and...

24 September 2020 4,937 4 View

Lysozymes extraction from Human saliva?

Need guidance related to isolate it through simple method. Suggest easiest and simplest methods.

17 January 2020 7,347 1 View

DNA Purification-Best Way?

Hello All, Could anyone please suggest me the best way to purify DNA from contaminants such as proteins, RNA, salts and particulates. The DNA was extracted manually from saliva samples therefore...

10 November 2019 3,559 2 View

Why we have to dilute saliva with buffer in the use of lateral flow assay?

Could you tell me about sample issue? or article that explained about this issue. I want to know the reason of saliva dilution. Why we can't use saliva sample as it is...

09 October 2019 4,437 1 View

How many participant for saliva cortisol test?

Hi, I plan to use the saliva cortisol sampling via the diurnal pattern of salivary cortisol to indicate stress together with the Perceived Stress Scale for stress level and mental wellbeing, the...

08 September 2019 2,841 1 View

Has anyone used Zymo DNA RNA shield for extractions from saliva?

I want to collect saliva samples in the Zymo DNA RNA shield in order to extract human DNA and RNA from the saliva. Has anyone tried this, either DNA, RNA or preferably both, and was the quality...

08 September 2019 7,806 1 View

Can anyone help me for the extraction of DNA from human saliva?

I have performed this experiment several times but failed.The main problem is that its UV spectra has a lot of distortion and its peak is not on 260nm. The DNA which i extracted has 1.5...

07 August 2019 1,941 3 View