Yasha N. B.

5 Questions 10 Answers 0 Followers

Questions related from Yasha N. B.

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

28 February 2021 863 3 View

Why am I getting 2 bands for Gibson Cloning Vector PCR?

Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...

12 January 2021 9,766 2 View

I got cerulean gene sequenced using universal T7 promoter and terminator primers. Why Fwd primer showed mismatches while Rev did not?

The question is based on Sanger sequencing results. Gene size is 717bp which falls within the Sanger range. I was curious as to why did one of the reactions of the same sample show odd results...

26 February 2020 8,242 6 View

Difference between Universal Primers and Degenerate Primers?

Also provide any link to good explanation of degenerate primers. Thank you.

28 January 2016 4,020 10 View

Bioinformatics and Molecular Biology - Research problems, Troubleshooting, and Solutions

This discussion is to bring together bioinformaticians and molecular biologists so that they can discuss research problems and share solutions. Thank you.

01 January 1970 8,667 5 View