I am using the cytoskeleton bk029 kit. It is suggested to snap freeze the proteins with liquid nitrogen. The problem is that I am left with nothing and there are 3 MAP fractions left. Is there any way to get around this problem?
If you’ve run out of liquid nitrogen and can’t snap freeze your protein samples using the Cytoskeleton BK029 kit, here are alternative methods to preserve your samples:
1. **Dry Ice**: Use dry ice to quickly cool your samples to around -78.5°C, which can effectively snap freeze proteins. Ensure samples are insulated to avoid direct contact with dry ice.
2. **-80°C Freezer**: Store your samples in a -80°C freezer if available. While not as rapid as snap freezing, this will help maintain protein integrity over time.
3. **Ice Water Bath**: Use an ice-water bath to temporarily cool samples before placing them in a freezer. This can slow enzymatic activity until you find a better solution.
4. **Liquid Nitrogen Alternatives**: Consider borrowing liquid nitrogen from nearby labs if you have access.
5. **Protein Stabilizers**: Add stabilizers like glycerol or DMSO to your samples before freezing to help maintain protein structure and function.
6. **Short-term Refrigeration**: If you need to use the fractions soon, keep them on ice in a refrigerator.
Always refer to your kit’s manual for any specific recommendations regarding these methods.
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