Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Best FE Program for Concrete Strengthened with FRP Sheets?

Colleagues, I have been using ANSYS to model concrete strengthened FRP sheets, but less than happy with it. Any has recommendation for efficient, easy to use FE program for modelling concrete...

22 February 2021 7,528 3 View

The type of filter paper used to filter an epoxidized oil (the mixture is an epoxidized oil with magnesium sulfate) ?

I have a mixture of epoxidized oil and magnesium sulfate wich type of filtre paper should i use

21 February 2021 3,944 3 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

How to dissolve querceitin in HEPES?

I am trying to do the isothermal titration calorimetry study of quercetin. I want to reach a final concentration of 5 mM quercetin in HEPES or MES buffer with

15 February 2021 2,159 3 View

What Buffer should i use for T4 virus for electron microscopy ?

Hello, I currently have my T4 virus in HEPES buffer. Unfortunately, there are always crystals that are contaminating my solution (due to the buffer in which the Viruses are) when I observe them...

01 February 2021 400 3 View

Why is my protein not binding to my reversed-phase column?

Hello everyone! Short facts: I am trying to perform reversed-phase chromatography via a desalting column (Waters: Sep-Pak tC18 3cc) of an already purified hydrophobic protein to exchange the...

01 February 2021 5,471 15 View

Is there a certain way of making HEPES buffer w/ KCl?

I am trying to switch from NaCl to KCl in my buffer. As soon as I add 4M KCl solution to DI water and HEPES, it precipitates. Would heating the buffer alleviate the problem? Or should i add KCl to...

07 January 2021 1,104 1 View

Fluorescence Polarization: Non zero numbers for calibration curve?

Hi! I am doing FP/FA to study competitive binding interactions. I am currently doing experiments to determine the optimal buffer for my protein. From the literature, I am trying HEPES and tris...

06 December 2020 8,496 2 View

Could anyone help with a 2-NBDG experiment on C2C12 cells?

I have been working with the C2C12 myotubes and measured their glucose uptake activity using an indirect glucose uptake assay (measuring how much glucose is left in the medium after stimulating...

23 November 2020 2,118 3 View

Is it possible to use 20 mM HEPES buffer to dissolve my treatment?

I am planning my animal study and would appreciate it if someone can provide advice regarding this Is it possible to use 20 mM HEPES buffer to dissolve my treatment for in vivo study for...

27 September 2020 5,508 3 View

The best siRNA transfection protocol for SK-N-AS cells?

Hello, I am looking for protocols which would be effective in the neuroblastoma cell line SK-N-AS? Thank you.

12 September 2020 8,769 3 View

Composition of HEPES buffer solution?

Hi All, I want to make HEPES buffer solution and when I search for the composition I could find two different types of it. Can anyone help me which one is the correct method of HEPES...

08 September 2020 450 4 View