I am working on neuroblastoma cells and just wanted to optimize cell culture conditions. I just need to know whether RPMI 1640 with HEPES can be used or not. Thanks in advance.
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
Fatal error in MPI_Allreduce: Other MPI error, error stack: MPI_Allreduce(1628)......: MPI_Allreduce(sbuf=000002459254A180, rbuf=000002459F86A140, count=4851, MPI_DOUBLE_COMPLEX, MPI_SUM,...
09 August 2024 7,615 1 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Hello everyone, I am currently working on a research project that aims to integrate machine learning techniques into an open source SIEM tool to automate the creation of security use cases from...
04 August 2024 3,196 2 View
We have an original Alluaudite-Molybdate we want to test for catalysis and photocatalysis applications.
04 August 2024 2,261 1 View
Jump 2 is the 1st app scientifically developed to measure your jump height.
31 July 2024 8,194 0 View
Using SPSS, I made my edits in one bar chart — e.g., font type and size, hiding grid lines, and colours, namely: blue, orange, green, purple, and grey for 5 bars, respectively, etc. — and I saved...
29 July 2024 1,981 1 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
Hi everybody! I got some strange results when I tested mitoSOX RED on Bone marrow-derived Macrophages. According to the publications, bone marrow-derived macrophages(BMDMs) generally show...
03 June 2024 9,853 1 View
My buffer components consists of 150mM Nacl 5mM HEPES 20mM EDTA and volume is made up to 10ml by ultrapure water what is the osmolarity of this soln ?
16 May 2024 5,245 0 View
For making a general mammalian cell lysis buffer, I have been using HEPES as a component. However, I came across a paper where they have used Tris in place of HEPES while the rest of the...
28 April 2024 6,120 2 View
Hi, I have transfected my HEK293 cells with pcDNA3.1_plasmid of interest with EGFP by using lipotransfection method. Post transfection 48h the egfp expression looks good with more than 50%...
20 April 2024 2,469 2 View
We are implementing a grant related to the study of supertargets in secondary cultures of human neuroblastoma cells - IMR-32, Kelly, SH-SY5Y, SK-N-AS etc. Comparing gene expression between cell...
16 April 2024 7,706 2 View
I am been working for a couple of months without success on setting up an assay based on GTPases loading with bodipy gdp and then measure the exchange to GTP in presence of various GEFs. Reading...
16 March 2024 1,600 3 View
I am working with SH-SY5Y cells and differentiating them into neurons using retinoic acid. I need to extract protein from them in order to conduct a western blot, however, I am having trouble...
31 January 2024 5,056 3 View
We have expressed our protein in chloroplast when we add extraction buffer it get precipitate soon after centrifugation as this is real quick even we don't have a time to prepare sample for...
10 January 2024 9,727 10 View
Hello, even though 7 days have passed since we thawed the newly ordered cells, they are still in a round shape structure. We are using Lonza EBM-2 and we supplemented the media with: Fetal Bovine...
10 December 2023 8,666 0 View
Hallo "Mein Protein ist heute...
08 November 2023 6,868 1 View