I've always blocked membranes for WB with 3-5% milk or BSA in PBST depending on the phosphorylation of protein to investigate. However, I am now reading in a protocol that they used the "Roti block" solution for blocking the membranes and I wonder why. Can I use the usual milk or BSA blocking solution instead of Roti block?
Thank you
Of course, cows of Holstein breed . Milk yield per lactation reaches 30,000 kg.
Yes, You Can use the usual milk or BSA blocking solution. In Western blot, after immobilizing the sample on a surface, a blocking buffer is used to cover the free sites that are not occupied by the antigen. This prevents antibodies or other proteins from adhering nonspecifically, reducing background noise and improving the accuracy of the assay. Examples of common blocking buffers: BSA (Bovine Serum Albumin). Skimmed milk (casein). Gelatin. Commercial products such as Blocking Solution (ROTI®Block), SuperBlock™, etc.
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