Silvia Granata

2 Questions 1 Answers 0 Followers

Questions related from Silvia Granata

Roti block instead of milk or BSA blocking?

I've always blocked membranes for WB with 3-5% milk or BSA in PBST depending on the phosphorylation of protein to investigate. However, I am now reading in a protocol that they used the "Roti...

27 January 2025 3,516 2 View

Which buffer should I use to aliquot antibody in?

for western blot analysis the buffer in which the antibody already is, written in the datasheet, or should I use already the bsa or milk solution that I use for final diluition?

18 November 2020 5,167 3 View