Hi there,
We'd like to extract DNA from microbes living in soybean, wheat and sugarcane roots, but we can't use liquid nitrogen by now for grinding some of these hard materials. Also gringing with a lysis buffer should be our last option given the number of samples. So I was wondering in other ways of breaking root cells. Did someone ever tried other ways like using beads and shaking, for example.. or other?
Many thanks
look t:
Brunner, I. , Brodbeck, S. , BÜchler, U. and Sperisen, C. (2001), Molecular identification of fine roots of trees from the Alps: reliable and fast DNA extraction and PCR–RFLP analyses of plastid DNA. Molecular Ecology, 10: 2079-2087. doi:10.1046/j.1365-294X.2001.01325.x
and
Article Protocol for isolation of genomic DNA from dry and fresh roo...
Thank you Muhammad and Alex. Going to have a look on your suggestions.