The pubococcygeus muscle or PC muscle is a hammock-like muscle, found in both sexes, that stretches from the pubic bone to the coccyx (tail bone) forming the floor of the pelvic cavity and supporting the pelvic organs.
I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...
03 March 2021 8,066 4 View
I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...
02 March 2021 1,830 3 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View
I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??
28 February 2021 2,153 1 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...
28 February 2021 6,560 14 View
Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...
27 February 2021 5,028 2 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...
22 February 2021 8,023 1 View
does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?
21 February 2021 6,483 3 View
We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...
13 February 2021 9,174 1 View
I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...
31 January 2021 8,324 1 View
I'm planning to excise mouse muscle to use for ex vivo mechanical testing. What is the best way to prevent the stiffening effects of rigor mortis from confounding results if I cannot isolate the...
30 January 2021 1,641 2 View
Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...
30 January 2021 9,535 2 View
Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...
28 January 2021 8,765 4 View
On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...
27 January 2021 608 2 View
Hello I am growing MSCs cells in either 24 wells or 12 wells plate and then I need to lysate these cells for RNA extraction I ususally get RNA from 5 to 30s ng/ul Is there is any idea or tips...
26 January 2021 8,531 3 View
Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo
26 January 2021 4,165 3 View