Farides Saavedra

2 Questions 2 Answers 0 Followers

Questions related from Farides Saavedra

RNAseq from fixed T cells?

Hello everyone, I would like to know if anyone has experience in performing RNAseq from lymphocyte suspension (Sorter, MACs, etc.) from fixed and permeabilized samples? I was thinking of sorting...

06 February 2024 2,882 0 View

Can I re-centrifugate a pseudotyped lentiviral particles with a sucrose cushion?

Hi, I'm trying to produce pseudotyped lentiviral particles but I need high titers of lentiviral particles. I used ultracentrifugation for this purpose with a sucrose cushion, and the pellet it was...

14 September 2016 7,048 2 View