RNase inhibition by SDS?

More Irina Panteleeva's questions See All

How to prevent detection HA-tagged protein after IP if I look for other proteins?

Hello, I do IP using HA-tagged beads. One of my proteins through which I precipitate complex has HA-tag. I see this protein (with HA-tag) after IP. The signal is strong and bright. The complex...

28 March 2024 4,493 2 View

Can I simultaneously measure concentration of cytokines in serum and culture medium with Multiplex Luminex assay?

Dear colleagues, We are planning to use Human XL Cytokine Base kit for evaluation of concentration of several cytokines. The plan was to measure concentration of cytokines both in blood serum, and...

20 February 2024 7,692 0 View

What is the price (average) in USD of Microwave-assisted hydrodistillator with a 1000 mL flask?

I need to buy this equipment to obtain several essential oils.

18 February 2024 401 0 View

Can I perform re-extraction of RNA from eluted RNA (Column filter extraction protocol)?

I encounter the following problem: I want to send viral RNA for further analysis. As the receiver requires, I must process my samples according to a regular RNA extraction protocol and send the...

06 February 2024 7,465 4 View

Development of a smart refrigerator for 1C?

What are the main advantages and potential applications of a smart refrigerator developed on the 1C platform, and what functionality can be implemented using this platform?

29 November 2023 8,294 1 View

Can I use a hot-start polymerase (antibody blocked polymerase activity) for amplification of PCR products before sequencing?

I have the following question: I have a nested PCR protocol that uses a chemically modified hot-start polymerase. However, in the lab, I have a hot-start polymerase, but bounded to an antibody....

16 May 2023 6,906 2 View

Significant overall model in PROCESS SPSS but insignificant predictor effects?

Hello everyone! I have a question regarding results from PROCESS in SPSS. The overall model (model summary) is significant, however, the main effects of the predictors and their interaction are...

22 April 2023 8,485 4 View

Significant overall model in PROCESS SPSS but insignificant predictor effects?

Hello everyone, what does it mean when the overall model (model summary) in PROCESS in SPSS is significant, however all of the effects of the predictors and their interactions are not significant?...

20 April 2023 3,205 5 View

This question was deleted ?

[DELETED]

19 February 2023 7,602 3 View

Have you observed a similar disease Ulmus pumila in your cities and countries and do you have information on the pathogen?

In the city of Dnepr and Dnipropetrovsk region (Ukraine) many damaged trees Ulmus pumila are found in 2021 -2022. Other elm species are not damaged. These are mainly mature trees. Young trees are...

05 February 2023 4,371 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Is someone interested in testing some of our new Alluaudite compounds (Molybdate) in the fields of heterogeneous catalysis and photocatalysis?

We have an original Alluaudite-Molybdate we want to test for catalysis and photocatalysis applications.

04 August 2024 2,261 1 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View