RNase activity on agarose gel?

17 May 2022 0 5K Report

Hi everybody,

I want to perform a preliminary study to detect RNase activity on agarose gel and the protein I want to test has very high degree of purity (>99%): can you please suggest me a protocol to perform this experiment? Do I have to preliminarily treat the protein before incubating with RNA?

Thanks for the answers

PS. the protein buffer is 20mM TRIS, 100mM NaCl pH 8,5

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