Has anyone done that?

I used mirvana in some of my DS runs without problems with library quality etc. You have to be careful when you want to also isolate sRNA species-there is an extra protocol for that. However I would recommend a column-free based approach in order to eliminate any chance of introducing biases.

ok... Thanks a lot.

STill I am asking whether you have noticed any length bias of mRNA. How was your data? was it good. How was the genome coverage?

Genome coverage was and data quality were acceptable. But what influences these parameters is the method of polyA isolation and not so much the RNA prep approach