TCGA data analysis for the interaction of miRNA and mRNA?

01 February 2017 651 5 View

What are the possible reasons for the reduction in GFP cell population?

Hi all.. I am using a mouse cell line with a silenced GFP promoter in it... I have been doing RNAi screening and have identified a candidate gene that might be involved in silencing.. Once I made...

04 May 2015 1,565 7 View

Are you familiar with Cp values for 18S rRNA?

03 April 2015 1,009 29 View

Why or how does serum reduce the transfection efficiency?

02 March 2015 4,685 7 View

Is the increased viscosity of RKO cell culture media a contamination?

Hi.. I am culturing RKO cells in DMEM media (10% FCS and 1%PEN/STREP).. I recently noticed when I was splitting the cells that the old media was very viscous.. Does it mean that my culture is...

10 November 2014 7,985 10 View

Is there any particular reason for using HEK cells for lentiviral packaging?

Hi all, dor lentiviral packaging, I used both HeLa and HEK cells and I found out that packaging had occurred in HEK but not in HeLa. Is there any particular reason for that? Has anyone tried...

10 November 2014 5,123 8 View

How to get the Standard Deviation?

I had used Permutation in Plink to check the validity of my markers after 10000 permutations. I used the command --mperm 10000 for permutation. Is there a way to get the Standard Deviation of the...

02 March 2013 2,498 8 View

Bonferroni Correction

I am using plink software to create an assoc file to get the P values and the number of SNPs which are in lakhs. The minimum P value that I am planning to use is 0.05 but since there are multiple...

01 February 2013 1,053 10 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View