Anuroop Venkateswaran Venkatasubramani

9 Questions 47 Answers 0 Followers

Questions related from Anuroop Venkateswaran Venkatasubramani

TCGA data analysis for the interaction of miRNA and mRNA?

Hi all, I have a data-set from TCGA consisting of miRNA and mRNA (example files attached). I would like to find the interaction between these two datasets, as in the up-regulation and...

02 February 2017 703 5 View

What are the possible reasons for the reduction in GFP cell population?

Hi all.. I am using a mouse cell line with a silenced GFP promoter in it... I have been doing RNAi screening and have identified a candidate gene that might be involved in silencing.. Once I made...

05 May 2015 1,618 7 View

Are you familiar with Cp values for 18S rRNA?

Hi all, I am doing qPCR for my stable mouse cell line and I am using 18S rRNA as an internal control. The Cp values of my test samples (RT-ve) were nil, i.e. no Cp value, but in the case...

04 April 2015 1,064 29 View

Why or how does serum reduce the transfection efficiency?

Hello guys!! I just have a doubt and I hope someone could give me an explanation. I have been doing lentiviral transduction and lipofection for quite a while. In both cases, absence of serum is...

03 March 2015 4,767 7 View

RNA seq data for cancer cell lines?

Hello, I am looking for databases that has the RNA seq data for different cancer cell lines and control cell lines. I am basically looking at CRC cancer cell line. I am using GEO but I would...

11 November 2014 259 3 View

Is the increased viscosity of RKO cell culture media a contamination?

Hi.. I am culturing RKO cells in DMEM media (10% FCS and 1%PEN/STREP).. I recently noticed when I was splitting the cells that the old media was very viscous.. Does it mean that my culture is...

11 November 2014 8,032 10 View

Is there any particular reason for using HEK cells for lentiviral packaging?

Hi all, dor lentiviral packaging, I used both HeLa and HEK cells and I found out that packaging had occurred in HEK but not in HeLa. Is there any particular reason for that? Has anyone tried...

11 November 2014 5,208 8 View

How to get the Standard Deviation?

I had used Permutation in Plink to check the validity of my markers after 10000 permutations. I used the command --mperm 10000 for permutation. Is there a way to get the Standard Deviation of the...

03 March 2013 2,542 8 View

Bonferroni Correction

I am using plink software to create an assoc file to get the P values and the number of SNPs which are in lakhs. The minimum P value that I am planning to use is 0.05 but since there are multiple...

02 February 2013 1,104 10 View