May I know if there's any alternative to replace alcohol (ethanol and isopropanol wash) in RNA extraction from whole blood? Chaotropic salts such as GuSCN or GuHCL helps in the washing steps?
Hi, I am facing a temperature convergence issue in Silvaco Atlas. At the threshold voltage the temperature is not converging. I applied given models models consrh bgn FLDMOB HCTE MASETTI...
02 May 2024 3,059 0 View
In my ddPCR assay to check my specific edits the PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and Probe 2 (labelled with...
14 March 2024 7,010 1 View
Hello, Is there any alternative (cheaper) Bio-Rad AutoDG-compatible tips? The price for a pack of 20 boxes has become double in 2024. Thanks
12 February 2024 1,350 0 View
Need guidance and help in understanding and interpretation of some data regarding the components forces that we get from the dynamometer after the side milling (up and down) operation. After the...
06 February 2024 4,554 5 View
WHEN WE DONE THE ELEMENTAL ANALYSIS OF THE BLACK SPOT AREA THE CHROMIUM CONCENTRATION INCREASES. CAN ANYONE PLEASE TELL ME WHAT IS THE REASON BEHIEND THE INCREASE IN THE CHROMIUM CONCENTRATION...
02 February 2024 7,918 2 View
Hi, I am currently using Bio-Rad Automated Droplet generator. I am happy with the result. I get on average 19K to 21K droplets per reaction. However, I have also encountered 1 to 2 samples...
12 December 2023 9,091 3 View
I have designed a ddPCR assay to detect gene edits in my organism. The PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and...
29 November 2023 1,506 10 View
Can I get brief explanation about this this theorem, which is applied in venturi meter ?
25 October 2023 2,438 9 View
I am working on an Abaqus User element and have 4 degrees of freedom(3 displacement and 1 user-defined). I am working on a single element example, where I want to define the initial condition of...
18 May 2023 6,997 0 View
I am currently planning on performing metabolic flux analysis in plants using isotopic sulfur. but I am stuck on how to replace the normal sulfur-containing nutrients in MS media ( such as...
22 December 2022 1,539 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View