Dear all, I need to extract RNA from cochineal insects (4-7 mm by 3-5 mm); I have them both dried and fresh frozen in RNAlater. Can someone suggest a protocol for this? I've seen people suggest QIAgen RNeasy kits for aphid RNA isolation, would that work here too?
Thank you!
Emilie Dion
We use the QIAgen RNeasy mini kit on tiny pieces of insect tissue and it works well. The tricky step with small tissue is the homogenization. Covering the sample with beads and homogenize with a bullet blender worked well here.
2 votes
0 thanks
Gautier Richard
We already used Mirvana Total RNA Extraction kit on pea aphids embryos. It worked really well, even for subsequent RNA-sequencing.
As mentionned by Emilie Dion, the homogenization steps are very important to retrieve enough RNA.
0 votes
0 thanks
- Badges
- Science method
More Ekaterina Mikhalev's questions See All
Similar questions and discussions