RNA extraction from whole blood in frozen EDTA tube at -80?

06 September 2024 1 9K Report

Is there a protocol for RNA extraction from blood frozen at -80? It has been frozen for 3 weeks. Should I perform a wash to remove hemoglobin?

Péter Gyarmati

It really depends on what you are looking for, but expect your RNA to be fragmented and cells ruptured.

https://www.nature.com/articles/s41598-021-96567-2

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