Beatriz de Arco

4 Questions 2 Answers 0 Followers

Questions related from Beatriz de Arco

Using of carrier RNA for RNAseq?

¿Could doubling the carrier RNA input during manual extraction using the QIAamp Viral RNA Mini Kit lead to increased non-target sequences in metatranscriptomic sequencing of microbial communities?...

14 April 2025 6,099 2 View

RNA extraction from whole blood in frozen EDTA tube at -80?

Is there a protocol for RNA extraction from blood frozen at -80? It has been frozen for 3 weeks. Should I perform a wash to remove hemoglobin?

06 September 2024 8,639 1 View

Low RNA concentrations – Am I degrading the sample?

Hello everyone, I am performing RNA extractions from oropharyngeal swabs and sera using different kits: Qiagen, Invitek, PureLink by Invitrogen, which are for viral RNA extraction as they are what...

05 September 2024 8,446 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

07 August 2024 2,643 3 View