Riveroxaban and apixaban ki against fXI?

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Can I use alpha MEM with FBS for osteoclast differentiation? And will trypsin usage decrease the ability of Raw cells differentiation?

I am using murine sRANKL for osteoclast differentiation in Raw 264.7. I would like to know if the differential media will have FBS or not. And I have been using Trypsin digestion for subculturing...

17 October 2022 4,402 2 View

What is the microbial degradation pathway of Reactive Yellow 145 dye (RY145)?

Dear friends, My research topic is "Dye degradation or colour removal using constructed wetland technology (a biological treatment system)". I am using a reactive azo dye (Reactive Yellow 145,...

08 September 2021 8,532 1 View

Greetings.I would like to know whether there is any incidence of Citrobacter freundii in spoilage of fish?

Myself Anu Ruby Benny. My research topic is related to identification of specific spoilage bacteria in iced fish.

28 July 2021 1,685 1 View

Is receiving a review invitation of a paper that has already in the second revision normal?

I am new to the "reviewer world". I received an invitation to review a manuscript for one of the open access journal from IEEE. When I download and open the manuscript, I realized that seems...

20 May 2020 506 13 View

Researcher recruitment for research project on PCSK9 protein ?

Dear all, I am Benny Danilo Belviso, a researcher of the Institute of Crystallography of CNR with experience in structural biology and X-ray based methods for structural studies, such as X-ray...

10 March 2020 6,085 0 View

Looking for data about % of total + absolute volume of irrigation with reclaimed/treated wastewater in different countries. Can you help?

I'm looking for reliable and recent data about level (% of total + absolute volume) of irrigation with reclaimed/treated wastewater in different countries/states. Can you help?

08 August 2019 6,493 3 View

Ligand doking in a volume defined by ccp4 map?

Dear all, I would to perform ligand docking in a volume that is defined by a ccp4 electron density map (Fo-Fc) at a given sigma instead of a standard regular grid. Do you know some software that...

30 May 2019 2,668 2 View

Error in plotting gain versus frequency in CST,solution?

When I do the evaluation in postprocessing I get the error- Template based post-processing result "Gain (IEEE),3D,Max. Value (Solid Angle)": Error in calling "Evaluate1D" function (No calculated...

18 December 2018 2,425 6 View

Can protein be absorbed on salt crystal?

Hello at all, I am trying to crystallize a protein by using standard sitting drop vapor diffusion setup and crystallization screens. After few days, spherulites or big needles aggregates appear in...

04 June 2017 8,083 7 View

dn/dc full lenght mAb?

I am using DLS measurements in order to calculate the second virial coefficient of a full-length mAbs in the presence of different buffer compositions and to do that, refractive-index increment...

03 April 2017 5,805 5 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View