Rippled or wavy bands in gel electrophoresis?

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If i want to test a particular compound/metabolite on my yeast strains ?

If i want to test a particular compound/metabolite on my yeast strains ( which have been transformed with the desired disease plasmid and shows the phenotype) . Then what is the method that I...

06 July 2018 3,182 0 View

Area under disease progress curve?

In plant pathology experiments generally disease severity measured in terms of grades of affected leaf area in relation to time is used for plotting the area under the disease progress...

05 June 2018 8,058 0 View

Which is better to suspend extracted DNA?

Which is a better one to suspend extracted DNA? TE buffer or ultrapure water?

05 June 2018 3,942 4 View

Long term storage of leaf tissue in CTAB buffer?

Can a leaf tissue be suspended in CTAB buffer until its DNA extraction? like for example if i go to a field and collect the leaf tissue and suspend the leaf tissues in CTAB in an eppendorf tube...

05 June 2018 1,717 1 View

CTAB DNA extraction method for insects?

IS CTAB buffer method of DNA extraction suitable for extracting insect DNA?

05 June 2018 8,074 2 View

Avoiding clumping of DNA ladder?

my 100 bp and 1kb ladder are not separating properly in gel inspite of providing the optimum agarose concentration, voltage and running time. the individual size bands dont spatially separate...

05 June 2018 1,179 0 View

Pre heating of CTAB buffer?

In what way does preheating of CTAB buffer prior to DNA extraction from leaf tissues improve the efficacy of DNA extraction or DNA recovery

05 June 2018 3,705 4 View

Preserving clones in glycerol stock?

I have cloned my virus in puc 18 vector. Right now Iam maintaining the clones by repeated subculture on lb medium amended with ampicillin. Is it possible to maintain the clones in glycerol...

04 May 2018 3,604 2 View

Increased manganese availability in flooded soils?

plants like paddy dont suffer from manganese deficiency since they are grown in flooded soils? what is the reason for the increased availability of manganese in flooded soils?

03 April 2018 1,618 3 View

Readymix of mastermix and long term storage?

Is it possible to make a readymix of PCR reaction cocktail involving components like water, dntps, taq polymerase etc. and keep it under long term storage for future use or should we use the mix...

03 April 2018 9,933 3 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

AUX gas reading problem on QE with full MS and PRM method in one run?

Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...

06 August 2024 4,953 0 View

People weight in Oaxaca Blinder Decomposition on R?

Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...

04 August 2024 6,033 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

How to perform EEG source analysis on each trial of data separately?

Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...

30 July 2024 2,744 1 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

How to use Desmond in HPC ?

Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...

28 July 2024 6,553 1 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View