Hi!
I am working on extracting RNA from Mycobacterium tuberculosis isolates under drug pressure for RNA seq. Can we use RNA elutes of RIN value ≥ 6 & rRNA 0.7?
How to enter the extinction coefficient in the calculation of the amount of anthocyanin?
15 June 2024 1,181 1 View
Call for Editors: TESOL Today TESOL Today, a distinguished scholarly journal at the forefront of Teaching English to Speakers of Other Languages (TESOL) and Applied Linguistics, is seeking...
08 May 2024 1,070 3 View
Dear TESOL and Applied Linguistics researchers, Greetings, TESOL Today invites educators, researchers, and practitioners in the field of Teaching English to Speakers of Other Languages (TESOL)...
02 May 2024 244 3 View
Practically it forms gel
10 March 2024 3,127 1 View
As i am doing multipass simulation with APDL Subroutines and at that time i had no idea how can i apply Element birth and death technique for simulation. And what logic should i used for the...
23 February 2024 3,833 0 View
study about evolution of skull of viperidae. some body help me about this.What studies have been done in this regard so far?
20 February 2024 4,804 0 View
We are looking for a research group that contains a research group in the field of management and human resources?
16 February 2024 9,102 6 View
I am working on a meta-analysis that examine the dose-response association of fasting plasma glucose (a continuous variable) on incident cardiovascular diseases. Although in most of the original...
06 February 2024 9,277 1 View
Does anyone know the latest DEA approaches to evaluate Environmental Efficiency? or what do you propose for that?
17 December 2023 5,871 2 View
I'd like to open a discussion on the correlation between particle size and the performance of pyrolysis. What are your insights regarding the influence of particle size on the efficiency and...
20 November 2023 8,816 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View