We are planning to induce chronic myeloid leukemia in mouse model with the MSCV-BCR-ABL-ires-GFP retroviral vector, but I am unable to find any company who is selling complete retroviral vector (MIG) with gene of interest (BCR-ABL). Does anybody know if any company sell MIG vector where we can order with specific target gene ?
or if after buying empty vector, we have to insert our gene of interest?
Addgene is selling the MSCV-IRES-GFP vector (http://www.addgene.org/20672/) for 65$. Therefore you've to try to clone the GOI of your own. It could be complicated because BCR-ABL is rather long and has a lot of restriction sites.
Another strategy could be the gateway cloning. Take a look at http://www.lifetechnologies.com/ and search for "gateway cloning" on the manufacturers website. If you've inserted your GOI into one pDONR vector it is very simple to shuttle the GOI into an appropriate expression vector. But the expression vector has to be an pENTR vector, with compatible sites. So take a careful look, if one of the pENTR-vector fullfills your wishes. Good luck!
Another option would be to contact authors of published papers, in which this construct was already used, and ask them to provide you with an aliquot.
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