How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Generate a structure file through PGDSpider?

Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...

29 December 2020 5,169 1 View

Transgenic or Mutagenic organisms, which is more stable genetically?

Hello, Which of these genetically altered organisms is more suitable for Scientific research? Is it the Transgenic hybrids or the Mutagenic organisms? Which is more genetically stable to be used...

16 December 2020 2,049 3 View

How to reconcile two sets of qPCR data from the same sample but acquired with different parameters (Ta, primer cc., etc.)?

17 November 2020 4,272 3 View

If we cross Diploid plant (Female Parent) x Tetraploid plant. How much chances of getting viable seed?

In case of guava crossing of Diploid (Psidium guajava, 2n = 22) with P. Molle (2n=44). Is there any chance to get viable seed set?

16 June 2020 9,317 3 View

Why do we add the same volume of PEG solution as MaMg+plasmid when performing gene transformation?

I'm doing GFP transformation using petunia protoplasts. I've tried 6 times and it seems I succeeded it for the first time. I guess it's important to keep cells alive so I changed the protocol a...

02 March 2020 711 1 View

Why do we add the same volume of PEG solution as MaMg+plasmid when performing gene transformation?

I'm doing GFP transformation using petunia protoplasts. I've tried 6 times and it seems I succeeded it for the first time. I guess it's important to keep cells alive so I changed the protocol a...

01 March 2020 9,616 1 View

How to normalize aequorin readouts for arabidopsis?

I have transgenic lines of arabidopsis expressing aequorin in the tonoplast, and am using them to determine calcium fluxes in week-old seedlings after certain treatments. The issue I am running...

17 January 2020 9,774 2 View

How to normalize aequorin readouts for arabidopsis?

I have transgenic lines of arabidopsis expressing aequorin in the tonoplast, and am using them to determine calcium fluxes in week-old seedlings after certain treatments. The issue I am running...

31 December 2019 2,127 2 View

Southern Hybridisation alternative/ QD PCR analysis for copy number?

Dear all, I am trying to find an alternative to southern hybridization for copy number detection in transgenic Arabidopsis. I have attempted the QD PCR protocol (T.Kihara ) for the protocol but I...

31 December 2019 4,566 3 View

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

26 June 2019 9,059 2 View