Hey Rachel,

I have never tried this protocol but have a look to the website. Worth give it a go if you cannot load another gel.

Good luck,

L.

http://www.scientistsolutions.com/forum/protein-detection-western-blots-gels-ip-protein-gel-electrophoresis-and-staining/destaining

You can re-stain without the need to destain. What I usually do is to start using the sensitizer and then repeat the protocol as usual. The only problem is that there will be a silver excess so that a precipitate will form.

If the purpose of the assay requieres to probe reproducibility I recommend you to repeat the whole electrophoresis.