Rachel Bell

4 Questions 4 Answers 0 Followers

Questions related from Rachel Bell

Repeating silver staining on same gel?

I would like to repeat a silver stain on a gel that I did not stain for long enough before adding the stop solution. Is it a requirement to destain for this purpose and if so, how is this best...

06 June 2016 1,528 2 View

Optimising a PCR reaction where I am currently getting no band?

I am in the process of preparing to do a RACE-PCR experiment and am testing some primers for this. I have designed one forward primer and two reverse primers (these will be used in a nested...

05 May 2016 1,723 3 View

What are your favourite western blot proliferation markers?

I have some cells that I know are proliferating less after drug treatment however are not showing a decrease in PCNA by Western Blot, I have read in a number of places now that PCNA can also be...

12 December 2015 9,673 4 View

Any suggestions on quantifying collagen prep isolated from rat tails?

I have followed a protocol to isolate collagen from rat tails to use in cell culture however I now need to quantify the collagen concentration and am having problems with this: In our lab we...

03 March 2015 9,636 8 View