I am trying to extract fungal DNA from clinical samples by using Guanidine thiocynate- phenol saturated . I got good DNA quantity but poor 260/230 ratio.
Whether repeatation of CI step can improve the ratio????
Repetition of ethanol washing will be able to remove phenol traces. Phenol traces are responsible for imbalance ratio of 260/280.
It is true that repeatation of ethanol washing results in good 260/280 ratio but it didn't improve 260/230 ratio
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