I have been trying to remove the vitreous humour from baby rat eyes (post natal day 15) and have been still having a lot of interference in my IHC staining. Does anyone have a good protocol to remove the vitreous from young eyes? I have found that the younger they are, the harder it is to get the vitreous off of the retina.
Thank you,
Rachel
I think you mean you have problem with autofluorescence? Do not you use something to eliminate the autofluorescence of blood vessels of any tissue? You can try with Lysine or milk. I do not have a protocol here right now, but it could not be difficult to find it.
Or... Do not you remove the lens during the processing of the tissue before slicing? Sometimes the vitreous comes out with the lens or at least it what happens when I do it in the shark.
Good luck!
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