Reliable anti-human IgA Antibody?

More Vladimir Khayenko's questions See All

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Cox regression baseline hazard?

Dear colleagues, I would like to make a mathematical equation of Cox regression model, however I am stuck with baseline hazard. I clearly understand interpretation of coefficients, but I can't...

24 February 2021 6,207 3 View

How to calculate the electric field in the rotating frame (the question in the general relativity)?

In the classical electrodynamics, there is one problem - to find the EM fields created by the charge moving with the constant velocity in the circular orbit or in the helical path (free electrons...

12 January 2021 3,689 1 View

Do you know anything about Elena Bokus, the early 20th Century Architect / Architectural Designer?

The available research of early 20th century history of architecture in North Macedonia, suggests that probably the first female architect / architectural designer was Elena Bokus (born in 1893...

23 December 2020 2,039 2 View

Can you recommend a short and free Deep Learning course that is sufficient to enable implementation on Java?

It would be best if the material is available on the web, a pdf version is also possible. If you still have knowledge of a good video course, please share.

29 November 2020 4,769 2 View

Why the Helmholtz decomposition theorem is not applicable to the electrodynamics?

My question is caused by one curious fact of the electrodynamics. It is commonly accepted opinion that the electromagnetic fields can at least formally be decomposed onto the rotational and...

10 September 2020 8,668 3 View

COS7 cell transfection - do you have a good protocol?

Dear all, lately we ran into trouble with COS7 cell transfection, we get low transfection rate with the protocol we use. Do you have a good transfection protocol? Preferably Calcium transfection...

23 May 2020 842 2 View

Can an electron and a hole have one common coordinate y_0 of the center of the quantum orbit in a two-dimensional semiconductor in a magnetic field?

Can an electron and a hole have one common coordinate y_0 of the center of the quantum orbit in a two-dimensional semiconductor in a magnetic field? (if they have the same or opposite directions...

16 January 2020 5,009 8 View

What is the density of excitons on a two-dimensional semiconductor in an external magnetic (and without magnetic) field?

What is the average density of excitons on a two-dimensional semiconductor in an external magnetic (and without magnetic) field? What is the dependence of this density on the magnetic field B?

12 January 2020 173 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

María Luisa Caballero

Hi Vladimir,

Are you referring to antihuman IgA antibodies?

In case this could help you I've used antibodies from SouthernBiotech for ELISA and they worked very well. Specifically in relation to IgA, I used the peroxidase-labeled antihuman IgA1, and it worked very well.

Vladimir Khayenko

Yes, specificity for Human IgA. Thanks!

Dmitry Zinovkin

Good day. Try to use Dako IgA antibody. It is anti-human. We have used it for detection IgA+ plasmocytes in colon cancer tissues.