Regarding Immunoaffinity Column Reuse?

01 January 1970 1 955 Report

Respected All We want to extract AFB1 after spiking in wheat flour. In the protocol, they mentioned the use of the IAC column, so my question is do we reuse the same column for different concentrations? Waiting for your positive reply.

Regards

Deepak Kala

I believe you can reuse it by regenerating the columns with high salt concentration buffers or low pH solutions, but the efficiency may suffer with repeated use.

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