Can someone please provide the reference sequence for the coagulation factor 8 gene? It is located on the long arm of the X chromosome (X-q28). I am trying to allign some primers to detect inversions in intron 22 and intron 1 which are responsible for the development of severe haemophilia A.
Hello Mihnea,
The easiest way would be to take the reference mRNA from NCBI database and BLAST it. You should retrieve the sequence of a BAC that contains the gene. You will need to reannotated the BAC sequence but should be good to go.
Best of luck
@Nicolas Vodovar: Hello! I will try, thank you for the quick response!
Hi Nicolas
you can also get all what you want on the UCSC website. take care when designing primers since this gene has a pseudogene with high homologies. therefore test your primers by an in silico PCR, or choose them in the non homologue regions (blast the F8 sequence in UCSC, you'll see what differs between both genes).
fred
- Badges
- Science topic
- Similar topics
- References