We wish to see if the endothelial glycocalyx is decreased in diabetic models and excess sodium models and we wanted to know if anyone had any suggestions for additive stains to standard TEM fixative?
I would appreciate any and all suggestions.
M.R. (Pete) Hayden MD
University of Missouri
Columbia, Missouri
Suggestion: use Lowicryl resin for embedding; carry out post-staining of sections with ruthenium red. I would expect high contrast. For details, ask Wolfgang Muss, Salzburg/Austria (also member of ResearchGate). We had discussions on this approach just a few days ago (s. Questions and Answers). He has also access to relevant literature.
Kind regards,
Frank Mayer
You can try to add 0.3% ruthenium red to standart fixation. See Arvid B. Maunsbach, Björn A. Afzelius
Biomedical Electron Microscopy: Illustrated Methods and Interpretations p. 44, it is available on Goggle.
Maybe this is helpful
Article Assessment and Imaging of the Cerebrovascular Glycocalyx
Request for PDF copy
Thank you Roel, Alexandra, Frank, Mohamed
All of your recommendations will be taken into consideration and I will
first use a Alcian Blue
the Alcian Blue8GX alone in immersion-fixation and non-perfused.
Alcian Blue 8GX +
2. Lanthanum that has been used and/or lanthanum plain without red.
in combination or maybe even +
3. ruthenium red
Also some have suggesteed
specifically
4. Lowicryl resin instead of standard acrylic resin -
it all will be trial and error..
YOUR THOUGHTs and recommendation have all been greatly appreciated.
M.R. (pete) Hayden
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining