What are good scanners for Western Blots that show a sensitivity similar to grey x-ray films?
Hi, I am looking for a commercially available antibody that detects pan STAT1 directly in blotted lysates and gives sensitive and clear cut results. Thank you for your help. Cheers, Jan
08 September 2018 4,693 1 View
I am interested to know how it compares to standard SDS-PAGE/Western Blotting procedures in terms of sensitivity (using grey x-ray films), separation quality, handling and overall costs. Any...
06 July 2014 5,950 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Require for Rietveld refinement in XRD.
08 August 2024 3,081 2 View
For example, I perform passivation process before electropolymerization of Py for adhesion to titanium surface. Is a similar or different procedure required for ITO? If necessary, I would be glad...
06 August 2024 3,176 0 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
What information we can get from PXRD analysis other than from SCXRD analysis of a crystal ?
30 July 2024 6,261 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
I will be doing an XRD on a sample which is powdered in natured and is a hydrogenated compound. I would like to know how we can understand the individual components and components by using the...
28 July 2024 4,548 2 View