Normality and equality of varience testing?

Hi guys, I have an experiemnt where three groups of fish were used, two of them were treated with two doses of virus, while the thrid is a control group. I want to compare the means of gene...

06 July 2019 3,604 5 View

Why my band after Coomassie blue is faint?

I am producing a recombinant protein in Rosetta, my protein is fused to MBP+his-tags at the amine terminus. I have solubilized it with sarkosyl. I am using IMAC column to purify my protein and...

02 March 2017 1,153 9 View

How to remove sarkosyl before mice immunization?

02 March 2017 6,614 2 View

How to prepare my proein for mice immunization?

02 March 2017 9,075 2 View

Can I use precipitated or aggregated r-protein for immunization?

I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...

02 March 2017 5,539 1 View

What 200 mM of NACL makes to MBP protein, binding or elution?

01 February 2017 9,511 7 View

My r-protein shows up in SDS-PAGE but not with western blot?

I am producing my MBP+his-tagged r-protein in Rosetta (75 kDa). I am using the anti his-tag column t purify my protein. I collected the sample flow-through after passing through the columns in...

01 February 2017 3,792 12 View

RNA isolation from fish skin samples ?

Hi guys, I want to ask about RNA isolation procedures from fish skin tissue samples? a full protocol would be appreciated.

02 March 2016 5,105 3 View

Is it tricky to use MS to sequence a small part of salmon IgT?

Is it tricky to use MS to sequence a small recombinant portion of salmon IgT?

31 December 2015 2,104 0 View

What is the result of MS (protein sequencing)?

I have a question regarding the result of MS (protein sequencing). We made the recombinant r- IgT (unique immunoglobulin in fish) in mammalian HEK cells, we want to characterize the r-protein, we...

11 December 2015 5,939 6 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View