Recombinant protein production??

More Haitham Tartor's questions See All

Normality and equality of varience testing?

Hi guys, I have an experiemnt where three groups of fish were used, two of them were treated with two doses of virus, while the thrid is a control group. I want to compare the means of gene...

06 July 2019 3,718 5 View

Why my band after Coomassie blue is faint?

I am producing a recombinant protein in Rosetta, my protein is fused to MBP+his-tags at the amine terminus. I have solubilized it with sarkosyl. I am using IMAC column to purify my protein and...

02 March 2017 1,285 9 View

How to remove sarkosyl before mice immunization?

is it necessary to remove the sarkosyl- my r-protein is solubilized in sarkosyl- before mice immunization?

02 March 2017 6,722 2 View

Can I use precipitated or aggregated r-protein for immunization?

I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...

02 March 2017 5,644 1 View

How to prepare my proein for mice immunization?

My r-protein (in e.coli) is only solublized in urea and I want touse it in a downsteam immunization procedure, dentaured protein is not convenient for production of Ab, how to solve this problem ?

02 March 2017 9,202 2 View

What 200 mM of NACL makes to MBP protein, binding or elution?

I have a question regarding to your Amylose resin kit https://www.neb.com/products/e8021-amylose-resin I read in the protocol...

01 February 2017 9,668 7 View

My r-protein shows up in SDS-PAGE but not with western blot?

I am producing my MBP+his-tagged r-protein in Rosetta (75 kDa). I am using the anti his-tag column t purify my protein. I collected the sample flow-through after passing through the columns in...

01 February 2017 3,961 12 View

RNA isolation from fish skin samples ?

Hi guys, I want to ask about RNA isolation procedures from fish skin tissue samples? a full protocol would be appreciated.

02 March 2016 5,239 3 View

Is it tricky to use MS to sequence a small part of salmon IgT?

Is it tricky to use MS to sequence a small recombinant portion of salmon IgT?

31 December 2015 2,236 0 View

What is the result of MS (protein sequencing)?

I have a question regarding the result of MS (protein sequencing). We made the recombinant r- IgT (unique immunoglobulin in fish) in mammalian HEK cells, we want to characterize the r-protein, we...

11 December 2015 6,059 6 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Do you know any references for analyzing stochastic fiber orientaion composites ?

Hello everyone I am looking for one or some books for propertes and behaviors of stochastic fibre orientation composites. unfotunately I could not find any suitable reference for thias by...

04 August 2024 3,461 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What's biological importance of bacteria &role of microbes improve crop productivity &soil health &role of bacteria as decomposers incycling nutrient?

What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?

31 July 2024 3,479 5 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

What is the role of microorganisms in soil fertility and crop production cycles and role of microorganisms in mineral transformation?

31 July 2024 8,483 8 View

How to get recent research on poultry production?

poultry production in nigeria

29 July 2024 5,518 2 View