7 Questions 18 Answers 0 Followers
Questions related from Hitesh Pawar
I have very little sample for western blot. I am comparing protein expression with some genes which showed good results in qRT PCR. But due to lack of protein sample I am able to do only 3-4...
02 February 2017 9,083 5 View
I have ELISA kit which have conc gradients from 16umol/l to 0.5 umol/L. and some kits have ng/ml so i want to make them equal in one concentration like all values in ng/ml (Mol Wt of that protein...
03 March 2015 3,010 1 View
Hi, I am using monoclonal antibody against B-actin but I got multiple bands in that blot. I tried 1:100, 1:500, 1:1000, 1:2000 and 1:5000 primary antibody concentrations, but still same thing...
01 January 2015 9,454 13 View
I am new in ELISA assays. I am using crude total protein to coat ELISA plates for Indirect ELISA using 100ng conc. of Ag. I already did titration for Ag and Ab's. I am not using any standard...
12 December 2014 6,230 6 View
I am doing ELISA test for some proteins to see the expression of it. I am also using B actin as endogenous control, but the values of average of OD of proteins with antibodies is in negative to B...
12 December 2014 9,401 9 View
We are putting ELISA for tissue samples. the protein amount which we coat at ELISA plate was 100 ng which is total extracted protein. So we take primary antibody and secondary and interpret...
12 December 2014 5,664 5 View
molecular biology
11 November 2014 5,474 16 View
