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I have very little sample for western blot. I am comparing protein expression with some genes which showed good results in qRT PCR. But due to lack of protein sample I am able to do only 3-4...
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I have ELISA kit which have conc gradients from 16umol/l to 0.5 umol/L. and some kits have ng/ml so i want to make them equal in one concentration like all values in ng/ml (Mol Wt of that protein...
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Hi, I am using monoclonal antibody against B-actin but I got multiple bands in that blot. I tried 1:100, 1:500, 1:1000, 1:2000 and 1:5000 primary antibody concentrations, but still same thing...
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I am new in ELISA assays. I am using crude total protein to coat ELISA plates for Indirect ELISA using 100ng conc. of Ag. I already did titration for Ag and Ab's. I am not using any standard...
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I am doing ELISA test for some proteins to see the expression of it. I am also using B actin as endogenous control, but the values of average of OD of proteins with antibodies is in negative to B...
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We are putting ELISA for tissue samples. the protein amount which we coat at ELISA plate was 100 ng which is total extracted protein. So we take primary antibody and secondary and interpret...
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molecular biology
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