Recipes for bacterial nitrogen-deficient liquid media?

08 April 2016 1 2K Report

Hi @all,

can you please provide me some recipes of bacterial nitrogen-deficient media, esp for cultivation of enterobacteria? What are your experiences? Which one works quite well in your lab?

Thanks a lot!

Arthur Goldstein

Hi Chirstian !

You can have a look at

Baldani, J. I., Reis, V. M., Videira, S. S., Boddey, L. H., & Baldani, V. L. D. (2014). The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant and Soil, 384(1–2), 413–431. https://doi.org/10.1007/s11104-014-2186-6

They give many recipes.

Best

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