Dear All,
Does anyone have the recipe for making mineral salt medium ?
I have found some information but i have few question.
Mineral salt medium (MSM) had the following composition (per liter): 2 g Na2SO4, 2.7 g (NH4)2SO4, 0.5 g NH4Cl, 14.0 g KHPO4, 3.6 g NaH2PO4 × H2O, 1.0 g (NH4)2 citrate [49]. After sterilization(Can I autoclave ???), the MSM was supplemented with sterile solutions of 100 µg/mL thiamine hydrochloride and 2 mL/L of trace element solution that included per liter: 0.5 g CaCl2 × 2H2O, 0.18 g ZnSO4 × 7H2O, 0.1 g MnSO4 × H2O, 20.1 g Na2-EDTA, 16.7 g FeCl3 × 6H2O, 0.16 g CuSO4 × 5H2O, 0.18 g CoCl2 × 6H2O [50]. MgSO4 was added to the final concentration of 2 mM at the start of the fermentation.
What is the advantage of using mineral salt medium over Terrific broth? I am planning to use CyDisCo plasmid co-transformed with my plasmid. The protein has 4 disulfide bonds.
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