Hi, all.
I am wondering if there is any chemogenetic or optogenetic AAV that can drive expression of receptors in active neuronal subtypes (e.g., CaMKII+ or GAD+).
I know that active populations might be "trapped" by combining
[ pAAV-cFos-Cre ] + [ pAAV-hSyn-DIO-{Receptor} ]
In this case, all cFos-positive neurons, including excitatory and inhibitory ones, should express receptors. I wonder if one can restrict the active population to a specific neuronal subtype.
I found [pAAV-CamKII-DIO-hM4D(Gi)-mCherry] (Plasmid #190240) that, in combination with AAV-cFos-Cre, does exactly what I want. Hovewer, I cannot find the same or similar for inhibitory neurons. I am not looking for chemogenetic-based AAV. It could be an optogenetic receptor. Or it could be based on the Tet-off/Dox system, not cFos/DIO. I just would like to know any possible methods for managing the activity of active sub-neuronal populations.
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