Hi there, anybody avare of eubacterial primers for real-time pcr, targeting ubiquitous housekeeping gene ? I not looking for 16S targeting primers. I need something elese, some protein coding gene. Thanks a lot for suggestions.
Hello Tomas,
I've attached a paper that might help - good luck!
Bacterial reference genes for gene expression studies by RT-qPCR: survey and analysis
Danilo J. P. RochaCarolina S. SantosLuis G. C. PachecoEmail author
Elhussein Mourad: I want measure (and compare) expression of certain genes in environments with different (and unknown) microbial composition and thus I do not want the results to be affected by 16S copy numbers. If I use protein coding housekeeping gene as a reference, I can roughly assume that there is usually 1copy per each genome. And I also need stable and roughly equal expression in all envionments.
Danielle Dillon:
Thank you, I will go through it:) ...and hope for luck:)
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