Tomas Chrudimsky

4 Questions 48 Answers 0 Followers

Questions related from Tomas Chrudimsky

Real-time primers for bacteria (other than 16S)?

Hi there, anybody avare of eubacterial primers for real-time pcr, targeting ubiquitous housekeeping gene ? I not looking for 16S targeting primers. I need something elese, some protein coding...

02 February 2017 5,250 3 View

How can I transfer and express a large gene cluster from one bacterium to another?

I need to take say 50kb gene cluster from the chromosome of one bacterial species and transfer it to another species. The gene cluster should be expressed. I want to observe if (how) the phenotype...

03 March 2014 4,645 12 View

Does anyone have experience with Mannose-resistant Type 1 fimbriae?

Does anybody have any experience in this field? I have a gammaproteobacterium and its genomic sequence and can see genes of type 1 fimbriae. I tried to agglutinate the yeast. The yeast...

12 December 2012 2,640 1 View

Symbiotic bacteria-freezing

I want to freeze and revive an insect symbiont. I grow it in MittsuhashiMaramorosh medium supplemented with 20% bovine serum at 27 degrees. It grows only on a layer of mosquito cells.I tried...

01 January 2011 1,009 3 View