Can we use distributed acoustic sensing in lab scale (~cm) applications?

I am looking for a way to increase the number of sensors in acoustic emission lab experiments. Fiber optics (DAS) is a natural candidate but the standard resolution is several meters. Is it...

20 September 2020 7,764 4 View

MHCI IP for MS what detachment agent to use?

Dear colleagues, We are planning to run some MHCI IP experiments to identify MHC-bound peptide in adherent cells. I was wondering what do you usually use to detach the cells? I found accutase as...

26 August 2020 2,931 1 View

What is the best way to obtain protein active center for quantum mechanic studies?

Hello there, I am going to model a simple reaction between Fe from heme of human erythrocyte catalase and hydrogen peroxide using QM methods. Before that I need to have valuable structure of...

13 August 2020 8,181 4 View

Which level of theory choose as a reference method?

Hello everyone, I do calculations on compounds with about 25-30 atoms (hydrogen, carbon, oxygen) and about 110 electrons in a water solvent (SMD solvation model). These are optimizations and...

05 June 2020 7,102 10 View

Are manufacturers obliged to identify all possible food contact surfaces for Listeria monocytogenes or an indicator organism testing?

Hi, Under 9 CFR 430, establishments in Alternative 2b and 3 are required to sample food contact surfaces for Listeria monocytogenes or an indicator organism....

10 March 2020 6,762 4 View

Why does Gaussian converts B3LYP to UB3LYP automatically?

Hello, I'm submit to Gaussian09 input file of molecule as in attached image. The problem is upon execution, when method is automatically changed from B3LYP (restricted by default) to UB3LYP and...

03 December 2019 4,626 2 View

Shipping Arabidopsis seeds to the USA?

I want to send the seeds of Arabidopsis mutants to the USA to my friend laboratory. Is it possible? And is it legal?

05 June 2018 4,545 3 View

What is the sequence of synthetic miRNeasy Serum/Plasma Spike-In Control cel-mir-39?

26 June 2017 4,629 8 View

How to calculate number of all particles in each cell in Fluent?

Hello, I would like to calculate number of all particles in one cell in during dpm simulation in fluent. Macro P_N(p) gives only number in single stream. Have anyone any idea?

10 May 2016 6,660 9 View

Any advice on a viscosity model for polyesterification phenomena?

To specialized in polyesterification: Does it exist any empirical equation that describes behaviour of viscosity changes as time-dependence function? To be precise, there is a precursor solution...

28 November 2015 7,356 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View