I am currently using two LNA probes (FAM and HEX) to quantitate two different SNPs at the same nucleotide position. Singleplex reaction for both probes yielded satisfactory efficiency for my standard. But when I tried running both probes on both SNPs template at different ratio (to test the sensitivity of the assay), I found that the probe signal for lower concentration SNP template was completely suppressed by the probe for higher concentration SNP template. However, both probe signal works fine when both SNP templates were at the same concentration. How do I optimize this?