Between Real time PCR and Nested PCR, which one is the best for enteroviruses detection in stool sample?
Nested pcr is fantastically sensitive and will detect and amplify very small amounts of viral dna in samples but is an end point procedure so will tell you the sequence exists but not how much there is. RT pcr uses more initial target dna but will tell you how much viral dna is present as well as confirming its presence. If I were testing huge numbers of samples I would use the cheaper nested pcr to show how many samples were positive and use rtpcr to study, for instance, the progress of viral excretion in stool
Conventional PCR (C-PCR) has been used to detect specific target genes in various microorganisms (5, 6, 13). Nested PCR (N-PCR) was developed to improve sensitivity but can give erroneous positive results due to DNA contamination @ Buhari Suraka
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